Explore CRISPR Cell Line Engineering by CellEDIT
With Our Workflow, We Overcome Cell Line Engineering Delivery Limitations.
The advent of CRISPR-Cas9 for targeted gene engineering has transformed the life sciences field. Despite its widespread application across various biological disciplines, challenges remain in engineering sensitive cells and introducing multiple edits.
At Cytosurge, we address these challenges with the CellEDIT Service and its unique workflow, beginning with the injection of a single cell. Our precise intra-nuclear injection technique minimizes off-targeting effect, ensuring highly controllable genetic modifications.
Partner with us for reliable, high-quality genetic edits.
Vector-free editing by intra-nuclear delivery
By directly injecting CRISPR reagents into the nucleus, our workflow eliminates the need for carriers or vectors and the constraints of template size. This approach saves time and reduces costs associated with designing complex plasmids.
Efficient on Hard-to-transfect cells
CellEDIT's gentle injection workflow uses FluidFM – Fluidic Force Microscopy, to deliver CRISPR complexes directly into the nucleus, making it ideal for developing cell lines in hard-to-transfect cells
Minimized Off-Targets
Direct intra-nuclear injection ensures that all CRISPR components are delivered simultaneously and at the right concentration into the nucleus, effectively minimizing off target effects.
Uses Cases - Engineered Cell Lines
Discover the effectiveness of CellEDIT firsthand—download our application notes and explore case studies showcasing its impact on immortalized and cancer cell lines.
Cancer Cell Lines
Find out how the CellEDIT workflow produced 3 monoclonal HPRT1 knockouts in SK-MES-1 cell, a notoriously hard-to-transfect cell line.
Immortalized Cell Lines
Explore how the CellEDIT workflow generated 5 monoclonal Hprt knockouts in C2C12 cell line through direct intra-nuclear injection of only 51 cells.
CRISPR technology facilitates precise genetic modifications, ensuring cell lines display consistent phenotypic and genotypic traits, which are essential for reproducible results and therapeutic advancements. This approach accelerates the creation of tailored cell models, greatly enhancing the speed and accuracy of biomedical discoveries.