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CellEDIT CRISPR Cell Line Engineering - Application note n*2
Overcoming the hard-to-transfect cell line hurdle
Key findings
- The CellEDIT workflow was used to successfully generate 3 monoclonal HPRT1 knockouts in SK-MES-1 cell, a notoriously hard-to-transfect cell line.
- Genotype analysis of 22 clones revealed that 55% (12) of these were edited at the target site and 23% (5) of all clones displaying editing on all four alleles.
- Gentle delivery of CRISPR-RNPs through intranuclear injection ensured a survival rate of 65% in SK-MES-1 cells, thereby showcasing the highly controllable and gentle nature of the CellEDIT workflow and its potential for editing notoriously hard-to-transfect cells.
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