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Live-seq: Live-cell Sequencing with Single-cell Biopsies
Our collaborators from ETH Zurich and EPFL Lausanne showcased Live-cell sequencing (Live-Seq) using single-cell cytoplasmic biopsies. They demonstrated that by using a FluidFM Nanosyringe, it is now possible to take an extract from a cell’s cytoplasm without killing it (Cell, 2016 [1]) and that this “single-cell biopsy” is a representable snapshot of a cell’s transcriptome at a given point in time (Nature, 2022 [2]).
Here we provide a brief summary of their findings:
- Taking a cytoplasmic biopsy with a FluidFM Nanosyringe preserves cell viability.
- Live-Seq enables stratification of different cell types and states and makes it possible to record short and long cell state transitions of the same cells.
- Direct correlation of a cell’s transcriptome to its phenotype enables visualization of minor transcriptional events in heterogeneous cell populations.
Find out more about Live-seq & cytoplasmic biopsies
Additional resources:
- The cytoplasmic biopsy solution with the FluidFM OMNIUM Platform
- The FluidFM OMNIUM Platform
- The FluidFM Technology
- LUTHOR HD: Pushing the Boundaries of Single Cell Transcriptome Analysis by in vitro mRNA Amplification.
- Download directly the blog post PDF version here.