What links CRISPR to Cancer Research?
The intersection of CRISPR-Cas systems and Cancer Research lies in cancer genetics. This encompasses the genetic mutations causing uncontrolled cell growth, the engineered cell lines modeling various cancer types, and targeted gene therapies for treatment.
Cancer Genes Identification
Researchers use CRISPR to delve deeper into the role of gene function or mutation in cancer progression. Several cancer types exist, but few share common genetic mutations. These mutations either activate a gene (oncogene) or deactivate a gene (tumor suppressor), resulting in uncontrolled cell growth.
Cancer Drug Targets Detection
Despite common mutations activating oncogenes or deactivating tumor suppressor genes, there are limited targetable genetic drivers for tumor initiation and progression, complicating the efficiency of cancer treatment. Nowadays, scientists use CRISPR screens to pinpoint potential cancer-causing genes or targets. Large-scale genetic or small molecule screens enable the assessment of numerous genetic mutations or molecules, aiding in the identification of cancer-related genetic mutations and cancer drug targets.
Cancer Disease Cell Models
CRISPR-Cas9 complexes are commonly employed to engineer cancer cell lines, which include:
Immortalized Cell Lines
Immortalized cancer cell lines, derived from tumors, can grow indefinitely in 2D cultures under specific conditions. They're frequently used in studying cancer biology and testing potential treatments due to their scalability and ease of use. However, their physiological relevance to actual cancer remains debated.
Primary Cell Lines
Primary cell lines, directly isolated from tissue, increasingly serve as model systems for cancer research. They more closely represent the original organism compared to continuously propagated cell lines.
3D Organoids
3D organoid cultures, offering a more comprehensive model, are used to replicate physiologically significant events in tumor formation. [2]
Perspectives in CRISPR Cancer Research
CRISPR/Cas technologies have been applied to disease modeling, gene therapies, transcription modulation, and diagnostics. [3] However, issues like potential immunological reactions and off-target effects persist. To address these, several innovative CRISPR/Cas tools and methods have emerged.
Off-target effects, resulting from unintended Cas9 protein cleavage in non-target sequences, have been a primary concern since CRISPR's inception. These effects can range from gene function loss to carcinogenesis. To mitigate this, scientists have employed bioinformatics tools (like gRNA design and off-target prediction software) and alternate biochemical components (such as Cas9 nickases and anti-CRISPR proteins). [4]
A notable advancement involves the controlled release of RNP complexes directly into the nucleus. This not only minimizes off-target effects but also curbs immune responses and cytosolic degradation, making it more efficient and precise than traditional methods.
