Explore CRISPR Cell Line Engineering with FluidFM
With Our Workflow, We Overcome Cell Line Engineering Delivery Limitations.
The advent of CRISPR-Cas9 for targeted gene engineering has transformed the life sciences field. Despite its widespread application across various biological disciplines, challenges remain in engineering sensitive cells and introducing multiple edits.
At Cytosurge, we address these challenges with the CellEDIT Service and its unique workflow, beginning with the injection of a single cell. Our precise intra-nuclear injection technique minimizes off-targeting effect, ensuring highly controllable genetic modifications.
Partner with us for reliable, high-quality genetic edits in 10 weeks.
Vector-free editing by intra-nuclear delivery
By directly injecting CRISPR reagents into the nucleus, our workflow eliminates the need for carriers or vectors and the constraints of template size. This approach saves time and reduces costs associated with designing complex plasmids.
Efficient on Hard-to-transfect cells
CellEDIT's gentle injection workflow uses FluidFM – Fluidic Force Microscopy, to deliver CRISPR complexes directly into the nucleus, making it ideal for developing cell lines in hard-to-transfect cells
Minimized Off-Targets
Direct intra-nuclear injection ensures that all CRISPR components are delivered simultaneously and at the right concentration into the nucleus, effectively minimizing off target effects.
Uses Cases - Engineered Cell Lines
Discover the effectiveness of CellEDIT firsthand — download our application notes and explore our case studies showcasing its impact on immortalized and cancer cell lines.
Cancer Cell Lines
Discover how
the CellEDIT workflow was used to produce 3 monoclonal HPRT1 knockouts in SK-MES-1 cell, a notoriously hard-to-transfect cell line.
Immortalized Cell Lines
Explore how
the CellEDIT workflow was used to generate 5 monoclonal Hprt knockouts in C2C12 cell line through direct intra-nuclear injection of only 51 cells.
Multiplex Editing
Find out how
the CellEDIT workflow boosted the editing efficiency while preserving cell characteristics and functionality in single and multiple KO CHO-K1 clones.
CRISPR technology facilitates precise genetic modifications, ensuring cell lines display consistent phenotypic and genotypic traits, which are essential for reproducible results and therapeutic advancements. This approach accelerates the creation of tailored cell models, greatly enhancing the speed and accuracy of biomedical discoveries.
Resources
Educational
Technical
Introduction to CRISPR Knockout Gene Editing with CellEDIT
An Overview of CellEDIT' CRISPR Cell Line Development Services
CellEDIT' CRISPR Cell Line Development Workflow
CellEDIT's Engineered Cell Lines
CRISPR-Engineered U2OS Cell Line
CRISPR-Engineered MDA MB 231 Cell Line
CRISPR-Engineered A549 Cell Line
CRISPR-Edited Hek293 Cell Line
CRISPR-Engineered C2C12 Cell Line
Media & Downloads
On-Demand CellEDIT's CRISPR Cell Line Engineering Webinar
CellEDIT's Application Note n*1 - Introducing the CellEDIT Workflow
CellEDIT's Application Note n*2 - Overcoming the hard-to-transfect cell line hurdle
Open Access Publication featuring CellEDIT: Antony, Justin S., Anabel Migenda Herranz, Tahereh Mohammadian Gol, Susanne Mailand, Paul Monnier, Jennifer Rottenberger, Alicia Roig‐Merino et al. " Accelerated generation of gene-engineered monoclonal CHO cell lines using FluidFM nanoinjection and CRISPR/Cas9" Biotechnology Journal 19, no. 4 (2024): 2300505.