Discover CRISPR Gene Editing with FluidFM
Custom CRISPR Gene Editing Service through Direct Intra-nuclear Delivery
CRISPR gene editing precisely cuts DNA, leveraging natural repair mechanisms to modify specific genome locations. [1,2] This adaptability of CRISPR-Cas9 has ignited rapid advancements in diverse fields, from synthetic biology and human gene therapy to neuroscience and agriculture.
While several gene editing techniques exist, each faces the challenge of efficiently accessing the nucleus to modify the genome without harming the cell. Though CRISPR excels in scenarios needing multiple edits within a single cell line or organism, it sometimes falls short due to low HDR efficiency and off-target effects.
Presently, the CellEDIT workflow with the FluidFM technology enhances CRISPR's efficiency and versatility in various cell types and is pivotal for cell line engineering and development.
Leverage CellEDIT's expertise to enhance your research capabilities: in 10 weeks, we create for you, custom and monoclonal knockout cell lines through direct intra-nuclear injection, suited for your project needs.
Vector-free Gene Editing
by direct intra-nuclear delivery.
Our workflow directly injects CRISPR reagents into the nucleus, removing the need for carriers or vectors and bypassing template size limitations.
Minimized Off-Targets
Direct intra-nuclear injection delivers all CRISPR components simultaneously and at the correct concentration, effectively minimizing off-target effects.
Suited for
Hard-to-Transfect Cells.
CellEDIT's gentle injection workflow uses FluidFM to deliver CRISPR complexes directly into the nucleus, suited for developing cell lines in hard-to-transfect cells.
CRISPR Gene Editing - Case Studies
Experience the efficiency of the CellEDIT workflow firsthand. Download our application notes and delve into compelling case studies that demonstrate its transformative impact on immortalized and cancer cell lines.
Cancer Cell Lines
Discover how
the CellEDIT workflow achieved 3 monoclonal HPRT1 knockouts in the notoriously hard-to-transfect SK-MES-1 cell line.
Immortalized Cell Lines
Explore how
the CellEDIT workflow generated 5 monoclonal Hprt knockouts in the C2C12 cell line by directly injecting only 51 cells.
Multiplex Editing
Find out how the CellEDIT workflow boosted the editing efficiency while preserving cell characteristics and functionality in single and multiple KO CHO-K1 clones.
Resources
Educational
Technical
Introduction to CRISPR Knockout Gene Editing with CellEDIT
An Overview of CellEDIT' CRISPR Cell Line Development Services
CellEDIT' CRISPR Cell Line Development Workflow
CellEDIT's Engineered Cell Lines
The CellEDIT Service - Custom Cell Line Engineering
CRISPR-Engineered U2OS Cell Line
CRISPR-Engineered MDA MB 231 Cell Line
CRISPR-Engineered A549 Cell Line
CRISPR-Edited HEK293 Cell Line
CRISPR-Engineered C2C12 Cell Line
Media & Downloads
On-Demand CellEDIT's CRISPR Cell Line Engineering Webinar
CellEDIT's Application Note n*1 - Introducing the CellEDIT Workflow
CellEDIT's Application Note n*2 - Overcoming the hard-to-transfect cell line hurdle
Case Study - Streamlined U2OS Cell Line Modification with the CellEDIT Service Workflow, featuring our CellEDIT Customer: Dr. Kanstantsin Siniuk.
Open Access Publication featuring CellEDIT: Antony, Justin S., Anabel Migenda Herranz, Tahereh Mohammadian Gol, Susanne Mailand, Paul Monnier, Jennifer Rottenberger, Alicia Roig‐Merino et al. " Accelerated generation of gene-engineered monoclonal CHO cell lines using FluidFM nanoinjection and CRISPR/Cas9" Biotechnology Journal 19, no. 4 (2024): 2300505.
References
[1] Jinek, Martin, et al. "A programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity." science 337.6096 (2012): 816-821.
[2] Li, Hongyi, et al. "Applications of genome editing technology in the targeted therapy of human diseases: mechanisms, advances and prospects." Signal transduction and targeted therapy 5.1 (2020): 1-23.