CellEDIT - CRISPR-Edited HEK293 Cell Line
Let us support you with a custom and high-quality engineered HEK293 cell line.
We’ll handle the CRISPR editing of your HEK293 cells, so you focus on your research
HEK293 is an epithelial cell line derived from the kidney of a human embryo. It is widely used in industrial biotechnology and toxicology research, with applications in efficacy and virucide testing.
HEK293 Cell Line Information
Organism
Human
Tissue Type
Kidney; Embryo
Cell Type
Epithelial
Donor Information
Age
Fetus
Gender
Unknown
Ethnicity
Unknown
HEK293 Cell Line Applications
Protein Expression: HEK293 cells are widely used to produce recombinant proteins, including therapeutic proteins and monoclonal antibodies. This is due to their high transfection efficiency and ability to perform human-like post-translational modifications.
Gene Function Studies: Researchers utilize HEK293 cells to study gene expression, regulation, and the functional roles of specific genes, making them a valuable tool for genetic research
Drug Screening and Development: HEK293 cells are employed in high-throughput screening assays to test the efficacy and toxicity of new drug candidates, facilitating the drug discovery process.
Viral Vector Production: These cells are commonly used to produce viral vectors, such as adenoviruses and lentiviruses, which are essential tools in gene therapy and genetic research.
Signal Transduction Research: HEK293 cells are used to investigate cellular signaling pathways, helping to elucidate the mechanisms of signal transduction and their implications in various diseases, including cancer and neurological disorders.
Protein-Protein Interaction Studies: As HEK293 cells are easily transfected, they are often used to investigate binary interactions of two ectopically expressed proteins. After transfection of the proteins of interest, the potential interaction are studied by co-immunoprecipitation studies.
Related Case Studies
Maximize your research capabilities with the help of CellEDIT. Custom engineered HEK293 cell lines are crucial in biological research due to their high transfection efficiency, making it ideal for producing recombinant proteins and studying gene function. It plays a significant role in drug discovery and development by enabling high-throughput screening of drug candidates. Additionally, HEK293 cells are essential for producing viral vectors used in gene therapy and genetic research. With the CellEDIT workflow and within 10 weeks, we can support you with custom and monoclonal knockout HEK293 cell lines, suited for your project needs.
Efficient on
Hard-to-transfect cells
Vector-free
direct intra-nuclear delivery
Minimized Off-Target
Immortalized Cells
Find out how
the CellEDIT workflow enabled the generation of 5 monoclonal HPRT knockout C2C12 cell lines through direct intra-nuclear injection of only 51 cells.
Hard-to-Transfect Cells
Discover how
the CellEDIT workflow facilitated the production of 3 monoclonal HPRT1 knockout SK-MES-1 cell lines, which are notoriously hard-to-transfect.
Available Edits using CellEDIT Engineered Cells
Knockout
Save time in the lab by empowering your research with CellEDIT. Confidently streamline your drug discovery workflow and investigate gene function with effective CRISPR knockouts.
Multiplex Editing
At CellEDIT, we provide you with multiplexed cell lines in 10 weeks. The efficiency and gentleness of our vector-free editing through intra-nuclear delivery, makes it an ideal system to perform multiplex gene editing in one go.
Related Resources
Educational
Technical
Introduction to CRISPR Knockout Gene Editing with CellEDIT
An Overview of CellEDIT' CRISPR Cell Line Development Services
CellEDIT' CRISPR Cell Line Development Workflow
CellEDIT's Engineered Cell Lines
CRISPR-Engineered U2OS Cell Line
CRISPR-Engineered MDA MB 231 Cell Line
CRISPR-Engineered A549 Cell Line
CRISPR-Engineered C2C12 Cell Line
CRISPR-Edited KPC Cell LineMedia & Downloads
On-Demand CellEDIT's CRISPR Cell Line Engineering Webinar
CellEDIT's Application Note n*1 - Introducing the CellEDIT Workflow
CellEDIT's Application Note n*2 - Overcoming the hard-to-transfect cell line hurdle
Open Access Publication featuring CellEDIT: Antony, Justin S., Anabel Migenda Herranz, Tahereh Mohammadian Gol, Susanne Mailand, Paul Monnier, Jennifer Rottenberger, Alicia Roig‐Merino et al. " Accelerated generation of gene-engineered monoclonal CHO cell lines using FluidFM nanoinjection and CRISPR/Cas9" Biotechnology Journal 19, no. 4 (2024): 2300505.