Staff Scientist Simona Patange  simona.patange@nist.gov

NIST, USA

Abstract:

Genome editing is a rapidly emerging biotechnology with the potential to transform many sectors of industry. For genome editing systems to achieve their maximum potential in research and commercial applications, it is critical to develop new measurement capabilities, control materials, and standards for evaluating genome-edited technologies and products (e.g., engineered cells) on their intended purpose. In this talk I give an overview of the U.S NIST Genome Editing Program and our efforts to solve current measurement challenges faced by the genome editing community. Single-cell manipulation and measurement technologies are valuable for increasing confidence in the genome editing process, and I present our use of FluidFM to investigate current measurement questions related to CRISPR/Cas9 formulation and delivery.