Abstract: The manipulation of micro-nano objects at the micro or nanoscale is technically essential to future nanodevice or nano-biotechnology development, multiple fields such as biochemistry analysis and nanoscale mechanical studies would be beneficial from that. The key element of micro-nano object manipulation is to generate a proper force field to trap and release the objects, however, the response of both hard and soft objects under confinement vortex-force fields is still missing but that is of great importance to further applications like nanoparticle delivery in the blood vessel or capillary. Herein, we utilize polystyrene nanoparticles (PS-NPs) and Chinese hamster ovary cells (CHO cells) as hard and soft-mater model systems respectively to study their behavior under the vortex force. As a result, PS-NPs were self-assembled into clusters driven by the interplay of multiple intermolecular forces, which were separated under the weak vortex force, only slippage of individual particles was observed. While the CHO cells were naturally attached to each other and strongly attached to the substrate, thus, they only get separated when applying strong vortex force, both slippage and rotation of individual cells were observed. In conclusion, the intensity of vortex force fields affected the form of matter movement and boundary condition. This study would be useful for understanding the matter movement under confinement conditions and pave the way for the mechanical biology study of in-vivo nanoparticles delivery and cancer cell metastasis.