Kinga Dóra Kovács    kovacs.kinga.dora@ek.hun-ren.hu

HUN-REN Centre for Energy Research, Hungary

Abstract:

In the past decade, extracellular vesicles (EVs) have attracted substantial interest in biomedicine. With progress in the field, we have an increasing understanding of cellular responses to EVs. In this Technical Report, we describe the direct nanoinjection of EVs into the cytoplasm of single cells of different cell lines. By using robotic fluidic force microscopy (robotic FluidFM), nanoinjection of GFP positive EVs and EV-like particles into single live HeLa, H9c2, MDA-MB-231 and LCLC-103H cells proved to be feasible. This injection platform offered the advantage of high cell selectivity and efficiency. The nanoinjected EVs were initially localized in concentrated spot-like regions within the cytoplasm. Later, they were transported towards the periphery of the cells. Based on our proof-of-principle data, robotic FluidFM is suitable for targeting single living cells by EVs and may lead to information about intracellular EV cargo delivery at a single-cell level.

Kovács, K.D., Visnovitz, T., Gerecsei, T., Peter, B., Kurunczi, S., Koncz, A., Németh, K., Lenzinger, D., Vukman, K. V., Balogh, A., Rajmon, I., Lőrincz, P., Székács, I., Buzás, E. I., & Horvath, R. (2023). Nanoinjection of extracellular vesicles to single live cells by robotic fluidic force microscopy. Journal of Extracellular Vesicles, 12, e12388.